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uea i dylight 649 vector laboratories  (Vector Laboratories)


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    Vector Laboratories uea i dylight 649 vector laboratories
    Uea I Dylight 649 Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 55 article reviews
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    (A) Representative immunofluorescence images of the OIR mouse retinal tissue at postnatal day 17 (P17), five days after intravitreal injection of PBS or iRECs. <t>Isolectin</t> <t>B4</t> (magenta), vaso-obliteration VO area (blue), and pathological neovascularization (NV) area (white). Scale bars: 300 µm. (B) Quantification of VO and pathological NV at P17. (C) Representative immunofluorescence images of whole mount retina, cross-sections of the OIR mouse retinal tissue, and 3D reconstructions of mouse vascular regions at P17 for PBS ( top ) and iREC ( bottom ) intravitreal injections. Mouse Isolectin B4 (red) and human UEA1 (green). Arrows depict pathological neovascular tufts and yellow crosshairs demonstrate location of xz and yz planes. Scale bars: 500 µm, 100 µm, and 25 µm, (left to right). (D) An immunofluorescence cross-sectional image of lumenized iREC vascular networks integrating with OIR mouse vasculature tissue on P17. Mouse Isolectin B4 (red) and human UEA1 (green). Asterisks denote lumens of human/mouse hybrid vasculature. Scale bar: 50 µm. (E) Quantification of mouse vascular network segment volume and lumen diameter across PBS and iREC injected groups. All quantifications are n = 8.
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    (A) Representative immunofluorescence images of the OIR mouse retinal tissue at postnatal day 17 (P17), five days after intravitreal injection of PBS or iRECs. <t>Isolectin</t> <t>B4</t> (magenta), vaso-obliteration VO area (blue), and pathological neovascularization (NV) area (white). Scale bars: 300 µm. (B) Quantification of VO and pathological NV at P17. (C) Representative immunofluorescence images of whole mount retina, cross-sections of the OIR mouse retinal tissue, and 3D reconstructions of mouse vascular regions at P17 for PBS ( top ) and iREC ( bottom ) intravitreal injections. Mouse Isolectin B4 (red) and human UEA1 (green). Arrows depict pathological neovascular tufts and yellow crosshairs demonstrate location of xz and yz planes. Scale bars: 500 µm, 100 µm, and 25 µm, (left to right). (D) An immunofluorescence cross-sectional image of lumenized iREC vascular networks integrating with OIR mouse vasculature tissue on P17. Mouse Isolectin B4 (red) and human UEA1 (green). Asterisks denote lumens of human/mouse hybrid vasculature. Scale bar: 50 µm. (E) Quantification of mouse vascular network segment volume and lumen diameter across PBS and iREC injected groups. All quantifications are n = 8.
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    Image Search Results


    (A) Representative immunofluorescence images of the OIR mouse retinal tissue at postnatal day 17 (P17), five days after intravitreal injection of PBS or iRECs. Isolectin B4 (magenta), vaso-obliteration VO area (blue), and pathological neovascularization (NV) area (white). Scale bars: 300 µm. (B) Quantification of VO and pathological NV at P17. (C) Representative immunofluorescence images of whole mount retina, cross-sections of the OIR mouse retinal tissue, and 3D reconstructions of mouse vascular regions at P17 for PBS ( top ) and iREC ( bottom ) intravitreal injections. Mouse Isolectin B4 (red) and human UEA1 (green). Arrows depict pathological neovascular tufts and yellow crosshairs demonstrate location of xz and yz planes. Scale bars: 500 µm, 100 µm, and 25 µm, (left to right). (D) An immunofluorescence cross-sectional image of lumenized iREC vascular networks integrating with OIR mouse vasculature tissue on P17. Mouse Isolectin B4 (red) and human UEA1 (green). Asterisks denote lumens of human/mouse hybrid vasculature. Scale bar: 50 µm. (E) Quantification of mouse vascular network segment volume and lumen diameter across PBS and iREC injected groups. All quantifications are n = 8.

    Journal: bioRxiv

    Article Title: Derivation of functional retinal endothelial cells from human pluripotent stem cells for therapeutics and modeling

    doi: 10.1101/2025.03.04.641453

    Figure Lengend Snippet: (A) Representative immunofluorescence images of the OIR mouse retinal tissue at postnatal day 17 (P17), five days after intravitreal injection of PBS or iRECs. Isolectin B4 (magenta), vaso-obliteration VO area (blue), and pathological neovascularization (NV) area (white). Scale bars: 300 µm. (B) Quantification of VO and pathological NV at P17. (C) Representative immunofluorescence images of whole mount retina, cross-sections of the OIR mouse retinal tissue, and 3D reconstructions of mouse vascular regions at P17 for PBS ( top ) and iREC ( bottom ) intravitreal injections. Mouse Isolectin B4 (red) and human UEA1 (green). Arrows depict pathological neovascular tufts and yellow crosshairs demonstrate location of xz and yz planes. Scale bars: 500 µm, 100 µm, and 25 µm, (left to right). (D) An immunofluorescence cross-sectional image of lumenized iREC vascular networks integrating with OIR mouse vasculature tissue on P17. Mouse Isolectin B4 (red) and human UEA1 (green). Asterisks denote lumens of human/mouse hybrid vasculature. Scale bar: 50 µm. (E) Quantification of mouse vascular network segment volume and lumen diameter across PBS and iREC injected groups. All quantifications are n = 8.

    Article Snippet: The retinas were then incubated with DyLight 649-conjugated Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 (Vector Laboratories) and Rhodamine-conjugated Ulex Europaeus Agglutinin (UEA 1, Vector Laboratories) overnight at 4°C in 5% normal goat serum containing 0.3% Triton X-100 in PBS.

    Techniques: Immunofluorescence, Injection